How to get a better PCR yield?

A)尽量去除杂质; B)可以把提取到的DNA用10mM Tris.Cl pH8稀释10-100倍，然后做PCR模板扩增。

A) Purify DNA as much as possible; B) Dependent on DNA concentration, dilute DNA 10-100 folds with 10 mM Tris.Cl pH8, use the diluted DNA as PCR template.